Orientia tsutsugamushi
Dzina la malonda
Zithunzi za HWTS-OT002B Orientia tsutsugamushiNucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Scrub typhus ndi matenda aacute febrile omwe amayamba chifukwa cha matenda a Orientia tsutsugamushi (Ot). Orientia scrub typhus ndi gram-negative obligate intracellular parasitic microorganism. Orientia scrub typhus ndi a mtundu wa Orientia mu dongosolo la Rickettsiales, banja la Rickettsiaceae, ndi mtundu wa Orientia. Scrub typhus imafala makamaka polumidwa ndi mphutsi za chigger zomwe zimanyamula tizilombo toyambitsa matenda. Ndi matenda yodziwika ndi mwadzidzidzi malungo, eschar, lymphadenopathy, hepatosplenomegaly, ndi zotumphukira magazi leukopenia, etc. Woopsa kwambiri, zingachititse oumitsa khosi, chiwindi ndi impso kulephera, zokhudza zonse Mipikisano chiwalo kulephera, ndipo ngakhale imfa.
Channel
| FAM | Orientia tsutsugamushi |
| Mtengo ROX | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | seramu yatsopano |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi / μL |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Analimbikitsa m'zigawo reagent: Macro & Micro-TestGeneralDNA/RNA Kit (HWTS-3019) (omwe angagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito reagent yochotsa izi. Voliyumu yotulutsidwa ndi 200µL, ndipo voliyumu yovomerezeka ndi100µl pa.
