Chitsanzo Chotulutsira Chitsanzo
Dzina la chinthu
Chotsukira Chitsanzo cha Macro & Micro-Test
Satifiketi
CE, FDA, NMPA
Zigawo zazikulu
| Dzina | Zigawo zazikulu | Chigawozofunikira | Kuchuluka |
| Chitsanzo Chotulutsachothandizira | Dithiothreitol, sodium dodecylsulfate (SDS), choletsa RNase,madzi oyera, opangidwa ndi surfactant | 0.5mL/Chidebe | Mbale 50 |
Dziwani: Zigawo zosiyanasiyana za zida sizingasinthidwe.
Momwe zinthu zimasungidwira komanso nthawi yosungiramo zinthu
Sungani ndi kunyamula kutentha kwa chipinda. Nthawi yosungiramo zinthu ndi miyezi 24.
Zida zogwiritsidwa ntchito
Zipangizo ndi zida zogwiritsira ntchito pokonza zitsanzo, monga ma pipettes, ma vortex mixers,malo osambiramo madzi, ndi zina zotero.
Zofunikira pa chitsanzo
Ma swab a m'kamwa omwe asonkhanitsidwa kumene, ma swab a m'mphuno.
Kulondola
Pamene chida ichi chikugwiritsidwa ntchito kuchotsa kuchokera ku CV yolondola ya mkati mwa nyumba ya ma replicates 10, coefficient of variation (CV, %) ya mtengo wa Ct si yoposa 10%.
Kusiyana kwa magulu osiyanasiyana
Pamene kulondola kwa mkati mwa nyumba kumayesedwa pa magulu atatu a zida zomwe zikuyesedwa popanga pambuyo pochotsa mobwerezabwereza, ndipo coefficient of variation (CV, %) ya mtengo wa Ct si yoposa 10%.
Kuyerekeza magwiridwe antchito
● Kuyerekeza bwino kwa kuchotsa
| Kuyerekeza bwino kwa mikanda ya maginito ndi njira yotulutsira chitsanzo | ||||
| kuganizira kwambiri | njira ya mikanda ya maginito | chitsanzo chotulutsa | ||
| orfab | N | orfab | N | |
| 20000 | 28.01 | 28.76 | 28.6 | 29.15 |
| 2000 | 31.53 | 31.9 | 32.35 | 32.37 |
| 500 | 33.8 | 34 | 35.25 | 35.9 |
| 200 | 35.25 | 35.9 | 35.83 | 35.96 |
| 100 | 36.99 | 37.7 | 38.13 | lembani |
Mphamvu yotulutsa chitsanzo cha sample releaser inali yofanana ndi njira ya maginito beads, ndipo kuchuluka kwa tizilombo toyambitsa matenda kungakhale 200Copies/mL.
● Kuyerekeza mtengo wa CV
| Kubwerezabwereza kwa kuchotsa chitsanzo chotulutsa | ||
| kuchuluka: 5000Makopi/mL | ORF1ab | N |
| 30.17 | 30.38 | |
| 30.09 | 30.36 | |
| 30.36 | 30.26 | |
| 30.03 | 30.48 | |
| 30.14 | 30.45 | |
| 30.31 | 30.16 | |
| 30.38 | 30.7 | |
| 30.72 | 30.79 | |
| CV | 0.73% | 0.69% |
Pamene anayesedwa pa makope 5,000 /mL, CV ya orFab ndi N inali 0.73% ndi 0.69% motsatana.
