Klebsiella Pneumoniae, Acinetobacter Baumannii ndi Pseudomonas Aeruginosa ndi Drug Resistance Genes (KPC, NDM, OXA48 ndi IMP) Multiplex

Kufotokozera Kwachidule:

Kiti iyi imagwiritsidwa ntchito pozindikira Klebsiella pneumoniae (KPN) mu vitro, Acinetobacter baumannii (Aba), Pseudomonas aeruginosa (PA) ndi majini anayi okana carbapenem (omwe akuphatikizapo KPC, NDM, OXA48 ndi IMP) m'zitsanzo za makoswe a anthu, kuti apereke chitsogozo cha matenda azachipatala, chithandizo ndi mankhwala kwa odwala omwe akuganiziridwa kuti ali ndi kachilombo ka bakiteriya.

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Dzina la chinthu

HWTS-RT109 Klebsiella Pneumoniae, Acinetobacter Baumannii ndi Pseudomonas Aeruginosa ndi Drug Resistance Genes (KPC, NDM, OXA48 ndi IMP) Multiplex Detection Kit (Fluorescence PCR)

Satifiketi

Epidemiology

Klebsiella pneumoniae ndi matenda ofala kwambiri omwe amayambitsa matenda opatsirana m'thupi ndipo ndi imodzi mwa mabakiteriya ofunikira kwambiri omwe amayambitsa matenda a nosocomial. Pamene mphamvu ya thupi yachepa, mabakiteriya amalowa m'mapapo kuchokera m'njira yopumira, zomwe zimayambitsa matenda m'mbali zosiyanasiyana za thupi, ndipo kugwiritsa ntchito maantibayotiki koyambirira ndiye njira yochiritsira.^[1].

Malo ofala kwambiri a matenda a Acinetobacter baumannii ndi mapapo, omwe ndi tizilombo toyambitsa matenda tofunikira kwambiri ku chibayo chopezeka kuchipatala (HAP), makamaka chibayo chogwirizana ndi Ventilator (VAP). Nthawi zambiri chimakhala ndi matenda ena a bakiteriya ndi bowa, omwe ali ndi zizindikiro za kuchuluka kwa matenda komanso kufa kwakukulu.

Pseudomonas aeruginosa ndiye kachilombo kofala kwambiri ka gram-negative bacilli komwe sikayambitsa kuyabwa m'matenda, ndipo ndi kachilombo kofunikira kwambiri ka matenda opatsirana omwe amapezeka kuchipatala, komwe kamakhala kosavuta kulowa m'thupi, kusinthasintha mosavuta komanso kukana mankhwala ambiri.

Njira

Dzina	PCR-Mix 1	PCR-Mix 2
Njira ya FAM	Aba	IMP
Njira ya VIC/HEX	Kulamulira Kwamkati	KPC
CY5 Channel	PA	NDM
ROX Channel	KPN	OXA48

Magawo aukadaulo

Malo Osungirako	≤-18℃
Nthawi yokhalitsa	Miyezi 12
Mtundu wa Chitsanzo	Kutupa kwa mbolo
Ct	≤36
CV	≤10.0%
LoD	1000 CFU/mL
Kufotokozera Mwapadera	a) Mayeso a cross-reactivity akuwonetsa kuti kit iyi ilibe reactivity yofanana ndi matenda ena opatsirana popuma, monga Streptococcus pneumoniae, Neisseria meningitidis, Staphylococcus aureus, Klebsiella oxytoca, Haemophilus influenzae, Acinetobacter jelly, Acinetobacter hemolytica, Legionella pneumophila, Escherichia coli, Pseudomonas fluorescens, Candida albicans, Chlamydia pneumoniae, Respiratory Adenovirus, Enterococcus ndi sputum samples zopanda zolinga, ndi zina zotero. b) Mphamvu yoletsa kusokoneza: Sankhani mucin, minocycline, gentamicin, clindamycin, imipenem, cefoperazone, meropenem, ciprofloxacin hydrochloride, levofloxacin, clavulanic acid, ndi roxithromycin, ndi zina zotero. kuti muyese kusokoneza, ndipo zotsatira zake zikusonyeza kuti zinthu zosokoneza zomwe zatchulidwa pamwambapa sizimasokoneza kuzindikira majini a Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa ndi carbapenem resistance KPC, NDM, OXA48 ndi IMP.
Zida Zogwiritsidwa Ntchito	Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio^®Machitidwe 5 a PCR a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka^®Dongosolo la PCR la 480 Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System