Fuluwenza A/B
Dzina la malonda
HWTS-RT003A Influenza A/B Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka fuluwenza A ndi matenda opatsirana opatsirana, omwe ali ndi mitundu ingapo monga H1N1 ndi H3N2, yomwe imakonda kusintha masinthidwe ndikufalikira padziko lonse lapansi.Kusintha kwa Antigenic kumatanthauza kusintha kwa kachilombo ka fuluwenza A, zomwe zimapangitsa kuti pakhale mtundu watsopano.Ma virus a fuluwenza B amagawidwa m'mibadwo iwiri ikuluikulu, Yamagata ndi Victoria.Ma virus a Influenza B amangokhala ndi antigenic drift, ndipo amazemba kuyang'anira ndi kuloledwa kwa chitetezo chamthupi cha munthu kudzera mu masinthidwe awo.Komabe, kusinthika kwa kachilombo ka fuluwenza B kumacheperachepera kuposa kachilombo ka fuluwenza A, komanso kachilombo ka fuluwenza B kungayambitsenso matenda amtundu wa kupuma kwa anthu ndikuyambitsa miliri.
Channel
| FAM | IFV A |
| Mtengo ROX | Ulamuliro Wamkati |
| VIC/HEX | IFV B |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | matenda a oropharyngeal |
| Ct | ≤28 |
| CV | ≤5.0% |
| LoD | IFV A: 500Copies/mL, IFV B: 500Copies/mL |
| Mwatsatanetsatane | 1. Cross-reactivity: palibe cross reactivity pakati pa zida izi ndi adenovirus mtundu 3, 7, human coronavirus SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, ndi HCoV-NL63, cytomegalovirus, enterovirus, parainfluenza virus, chikuku virus, human metapneumovirus, mumps virus, kupuma syncytial virus mtundu B, rhinovirus, bordetella pertussis, chlamydia pneumoniae, corynebacterium, escherichia coli, haemophilus influenzae, jactobacillus, morarabakteria catarrhalis, mycobacillus mycoplasmosis, myopia tidis , Neisseria gonorrhoeae, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, streptococcus pneumoniae, streptococcus pyogenes, streptococcus salivarius ndi DNA ya anthu genomic. 2. Mayeso osokoneza: Zinthu zosokoneza zimasankhidwa monga mucin (60mg / mL), magazi aumunthu, oxymetazoline (2mg / mL), sulfure (10%), beclomethasone (20mg / mL), dexamethasone (20mg / mL), flunisolide ( 20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL) ), azithromycin (1mg/L), cephalosporin (40μg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir phosphate (60ng/mL), ribavirin (10mg/L), ndi zotsatira zikusonyeza kuti zinthu zosokoneza zomwe zili pamwambazi zilibe chosokoneza pakuzindikira zida. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR dongosolo LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System ndi BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Analimbikitsa m'zigawo reagents: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (omwe angagwiritsidwe ntchito ndi Macro & yaying'ono-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitidwa molingana ndi IFU.Voliyumu yachitsanzo yochotsa ndi200μLVoliyumu yovomerezeka ya elution ndi 80μL.
Njira 2.
Ovomerezeka m'zigawo reagents: Macro & yaying'ono-Test Chitsanzo Release Reagent (HWTS-3005-8).Kuchotsa kuyenera kuchitidwa molingana ndi IFU.
Njira 3.
Ma reagents ofunikira: Nucleic Acid Extraction kapena Purification Kit (YDP315-R).Kuchotsa kuyenera kuchitidwa molingana ndi IFU.Voliyumu yotulutsa ndi 140μL.Voliyumu yovomerezeka ndi 60μL.
