Influenza A Virus Universal/H1/H3
Dzina la malonda
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit(Fluorescence PCR)
Epidemiology
Influenza virus ndi mtundu woyimira wa Orthomyxoviridae. Ndi tizilombo toyambitsa matenda timene timawononga kwambiri thanzi la munthu. Ikhoza kupatsira wolandirayo kwambiri. Mliri wa nyengoyi umakhudza anthu pafupifupi 600 miliyoni padziko lonse lapansi ndipo umayambitsa kufa kwa 250,000 ~ 500,000, pomwe kachilombo ka fuluwenza A ndiye kamene kamayambitsa matenda ndi kufa. Kachilombo ka Influenza A ndi RNA yokhala ndi nsonga imodzi yokha. Malingana ndi pamwamba pake hemagglutinin (HA) ndi neuraminidase (NA), HA ikhoza kugawidwa m'magulu 16, NA Ogawidwa mu 9 subtypes. Pakati pa mavairasi a chimfine A, ma virus a fuluwenza omwe amatha kupatsira anthu mwachindunji ndi awa: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 ndi H10N8. Pakati pawo, H1 ndi H3 subtypes ndizovuta kwambiri, ndipo ndizofunikira kwambiri kuziganizira.
Channel
| FAM | fuluwenza A universal mtundu virus nucleic acid |
| VIC/HEX | fuluwenza A H1 mtundu wa virus nucleic acid |
| Mtengo ROX | fuluwenza A H3 mtundu wa virus nucleic acid |
| CY5 | ulamuliro wamkati |
Technical Parameters
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | matenda a nasopharyngeal |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Makopi / μL |
| Mwatsatanetsatane | Palibe kupatsirananso ndi zitsanzo zina zopumira monga Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapnevirus A1/B2 syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, etc. ndi DNA ya anthu genomic. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems(Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Nucleic Acid Extraction or Purification Reagent (YDP315-R) yolembedwa ndi Tiangen Biotech (Beijing) Co., Ltd. The m'zigawo ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito. Voliyumu yachitsanzo yotengedwa ndi 140μL, ndipo voliyumu yovomerezeka ndi 60μL.
Njira 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). The m'zigawo ayenera kuchitidwa mosamalitsa malinga ndi malangizo ntchito. Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
