Kachilombo ka Fuluwenza A komwe kali konse/H1/H3
Dzina la chinthu
HWTS-RT012 Influenza A Virus Universal/H1/H3 Nucleic Acid Multiplex Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka fuluwenza ndi mtundu woyimira wa Orthomyxoviridae. Ndi tizilombo toyambitsa matenda tomwe timawopseza kwambiri thanzi la anthu. Tikhoza kufalikira kwambiri kwa munthu amene ali ndi kachilomboka. Mliriwu umakhudza anthu pafupifupi 600 miliyoni padziko lonse lapansi ndipo umapha anthu 250,000 mpaka 500,000, ndipo kachilombo ka fuluwenza A ndiye kamayambitsa matenda ndi imfa. Kachilombo ka fuluwenza A ndi RNA yokhala ndi chingwe chimodzi chokha. Malinga ndi hemagglutinin yake pamwamba (HA) ndi neuraminidase (NA), HA ikhoza kugawidwa m'magulu 16, NA Yogawidwa m'magulu 9. Pakati pa mavairasi a fuluwenza A, magulu a mavairasi a fuluwenza omwe amatha kufalikira mwachindunji kwa anthu ndi awa: A H1N1, H3N2, H5N1, H7N1, H7N2, H7N3, H7N7, H7N9, H9N2 ndi H10N8. Pakati pawo, magulu a H1 ndi H3 ndi oopsa kwambiri, ndipo ndi ofunika kuwaganizira kwambiri.
Njira
| FAM | Fuluwenza A mtundu wa kachilombo ka nucleic acid |
| VIC/HEX | chimfine cha mtundu wa H1 virus nucleic acid |
| ROX | chimfine cha mtundu wa H3 virus nucleic acid |
| CY5 | ulamuliro wamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | swab ya m'mphuno |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Kufotokozera Mwapadera | Palibe kuyanjana ndi zitsanzo zina za kupuma monga Influenza A, Influenza B, Legionella pneumophila, Rickettsia Q fever, Chlamydia pneumoniae, Adenovirus, Respiratory Syncytial Virus, Parainfluenza 1, 2, 3, Coxsackie virus, Echo virus, Metapneumovirus A1/A2/B1/B2, Respiratory syncytial virus A/B, Coronavirus 229E/NL63/HKU1/OC43, Rhinovirus A/B/C, Boca virus 1/2/3/4, Chlamydia trachomatis, adenovirus, ndi zina zotero. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Kuchotsa Nucleic Acid kapena Chotsukira (YDP315-R) chopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chochotsedwa ndi 140μL, ndipo kuchuluka koyenera kwa elution ndi 60μL.
Njira yachiwiri.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL, ndipo kuchuluka koyenera kwa elution ndi 80μL.
