Fuluwenza mu virus paliponse / h1 / h3
Dzina lazogulitsa
Hwts-rt012 fuluwenza chilombo kapena h1 / h3 nuclec acid placlex dip (fluorescence pcr)
Epidemiology
Kachisi wa fuluwenza ndi mitundu yoyimira ya orthomyxomtalidae. Ndilawezo zomwe zimawopseza kwambiri thanzi la anthu. Itha kupatsirana zowombera kwambiri. Mliri wapadera womwe umakhudza anthu pafupifupi 600 miliyoni padziko lonse lapansi ndipo amayambitsa kufa 250,000 ~ 500,000, komwe fuluwenza ndiye chifukwa chachikulu cha matenda ndi imfa. Fuluwenza ku virus ndi RNAS-RNARED STARD. Malinga ndi hemoglutinin (ha) ndi neuraminase (na Pakati pa fuluwenza, baza la ma virus a fuluwenza omwe amatha kupatsira anthu ndi: sh3n1, H3N2, H7N2, H7N7, H7n2 ndi H7N2. Pakati pawo, H1 ndi H3 Badeypes ndi wa pathogenic, ndipo makamaka ndi oyenera chidwi.
Ngalande
| Banja | fuluwenza chilengedwe chonse vission acid |
| Vic / Hex | fuluwenza ndi kachilombo ka H1 |
| Rox | fuluwenza mtundu wa h3 mtundu wa nyukiliya acid |
| Cy5 | kuwongolera kwamkati |
Magawo aluso
| Kusunga | ≤-18 ℃ |
| Alumali-Moyo | Miyezi 9 |
| Mtundu wofanana | nasopharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| Lodu | 500 makope / μl |
| Chifanizo | Palibe Chikumbutso cha Zithunzi Zina Zopumira monga, fuluwella b, a Legionella qoumophila, chlainflueonerza 1, ma virus a adnopie, metapneum, metaphneumor a1 / B1 / B2, Kupumira kwa kachilombo ka A / B, Connavirus 229E / NLU1 / Hku1 / OC43, Rhinorurrus A / B / ChLen, Rhinorururus A / B / Chlarmururus a / B. Chlamydia Trachiwatis 1/2, Rhinovirus A / B. Chlamydia Trachimo, ndi Chlamy |
| Zida Zogwirizira | Ntchito za bioshasystems 7500 pcr Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Ma pcr-5 a PCR Slan-96p Real PCR Systems (Hongshi Chaurchirol Technology Co., Ltd.) Speccycler®480 PCR System Stonegene 9600 kuphatikiza njira zenizeni za PCR (FQD-96A, maluso a bioriou bioer) Ma-6000 enieni ophatikizira cycler (Suzhou Molarray Colarray Colary Colarray Colary Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colary Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Colarray Cor Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Zilonda za Nucleic Cell kapena kuyeretsa (ydp315-r) ndi tiangen biotech (Beijing) Co., Ltd. Kuchotsa kuyenera kuchitika molingana ndi malangizo ogwiritsira ntchito. Buku la zitsanzo zoyendetsedwa ndi 140μL, ndi mafakitale olimbikitsidwa ndi 60μL.
Njira 2.
Macro & Bigro-Chefro-RNA Kit (HWTS-3004-32, HWTS-3004-48) ndi macro-3006c, 3006B). Kuchotsa kuyenera kuchitika molingana ndi malangizo ogwiritsira ntchito. Voliyumu yoyendetsedwa ndi 200μc, ndipo kuchuluka kwa Evest Evel ndi 80μl.
