Kusintha kwa majini a PML-RARA a anthu
Dzina la chinthu
HWTS-TM017AKiti Yodziwira Kusintha kwa Majini a PML-RARA ya Munthu (Fluorescence PCR)
Epidemiology
Acute promyelocytic leukemia (APL) ndi mtundu wapadera wa acute myeloid leukemia (AML). Pafupifupi 95% ya odwala a APL amatsagana ndi kusintha kwapadera kwa cytogenetic, komwe ndi t(15;17)(q22;q21), komwe kumapangitsa jini ya PML pa chromosome 15 ndi jini ya retinoic acid receptor α (RARA) pa chromosome 17 kuphatikizana kuti apange jini ya PML-RARA fusion. Chifukwa cha kusiyana kosiyana kwa jini ya PML, jini ya PML-RARA fusion ikhoza kugawidwa m'magulu awiri: long type (L type), short type (S type) ndi variant type (V type), zomwe zimakhala pafupifupi 55%, 40% ndi 5% motsatana.
Njira
| FAM | Jini lophatikizana la PML-RARA |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ Mumdima |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | mafuta a m'mafupa |
| CV | <5.0％ |
| LoD | Makope 1000/mL. |
| Kufotokozera Mwapadera | Palibe kusinthana kwa majini ndi majini ena osakanikirana a BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, ndi TEL-AML1. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chothandizira kuchotsa chomwe chikulangizidwa: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Kuchotsa kuyenera kuchitika motsatira IFU.
