Munthu PML-RARA Fusion Gene Mutation
Dzina la malonda
Chithunzi cha HWTS-TM017AHuman PML-RARA Fusion Gene Mutation Detection Kit (Fluorescence PCR)
Epidemiology
Acute promyelocytic leukemia (APL) ndi mtundu wapadera wa acute myeloid leukemia (AML). Pafupifupi 95% ya odwala APL amatsagana ndi kusintha kwapadera kwa cytogenetic, t (15; 17) (q22; q21), zomwe zimapanga jini ya PML pa chromosome 15 ndi retinoic acid receptor α gene (RARA) pa chromosome 17 yosakanikirana kuti ipange PML-RARA gene fusion. Chifukwa cha kusweka kosiyana kwa jini ya PML, jini ya PML-RARA yophatikizika imatha kugawidwa mumtundu wautali (mtundu wa L), mtundu waufupi (S mtundu) ndi mtundu wosiyana (mtundu wa V), wowerengera pafupifupi 55%, 40% ndi 5% motsatana.
Channel
| FAM | PML-RARA fusion jini |
| Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ Mumdima |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | fupa la mafupa |
| CV | <5.0％ |
| LoD | 1000 Makopi / ml. |
| Mwatsatanetsatane | Palibe kuyanjananso ndi mitundu ina yophatikizika BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, ndi TEL-AML1 gene fusion. |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR System Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Zomwe zimalangizidwa m'zigawo: RNAprep Pure Blood Total RNA Extraction Kit (DP433). Kuchotsa kuyenera kuchitidwa molingana ndi IFU.
