Kusintha kwa majini a BCR-ABL Fusion kwa Anthu
Dzina la chinthu
HWTS-GE010A-Human BCR-ABL Fusion Gene Mutation Detection Kit (Fluorescence PCR)
HWTS-GE016A-Human BCR-ABL Fusion Gene Mutation Detection Kit (Fluorescence PCR) youma ndi yozizira (Freeze-dryed Human BCR-ABL Fusion Gene Mutation Kit)
Epidemiology
Matenda a myelogenousleukemia (CML) ndi matenda oopsa a clonal a maselo oyambira a hematopoietic. Odwala oposa 95% a CML amakhala ndi Philadelphia chromosome (Ph) m'maselo awo amagazi. Matenda akuluakulu a CML ndi awa: Jini la BCR-ABL fusion limapangidwa ndi kusinthana pakati pa abl proto-oncogene (Abelson murine leukemia viral oncogene homolog 1) pa mkono wautali wa chromosome 9 (9q34) ndi jini la breakpoint cluster region (BCR) pa mkono wautali wa chromosome 22 (22q11); puloteni ya fusion yomwe imalembedwa ndi jiniyi ili ndi ntchito ya tyrosine kinase (TK), ndipo imayambitsa njira zake zotsatsira zizindikiro (monga RAS, PI3K, ndi JAK/STAT) kuti ilimbikitse kugawikana kwa maselo ndikuletsa apoptosis ya maselo, zomwe zimapangitsa kuti maselo azichulukana kwambiri, motero zimayambitsa CML. BCR-ABL ndi chimodzi mwa zizindikiro zofunika kwambiri zodziwira matenda a CML. Kusintha kwa mphamvu kwa kuchuluka kwa zizindikiro zake ndi chizindikiro chodalirika cha kutsimikizira kwa leukemia ndipo kungagwiritsidwe ntchito kuneneratu kubwereranso kwa leukemia pambuyo pa chithandizo.
Njira
| FAM | Jini yosakanikirana ya BCR-ABL |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | Madzi: ≤-18℃ Mumdima |
| Nthawi yokhalitsa | Madzi: miyezi 9 |
| Mtundu wa Chitsanzo | Zitsanzo za mafupa |
| LoD | Makope 1000/mL |
| Kufotokozera Mwapadera
| Palibe kusinthana kwa majini ndi majini ena osakanikirana TEL-AML1, E2A-PBX1, MLL-AF4, AML1-ETO, ndi PML-RARa |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio® 5 a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
