HPV16 ndi HPV18
Dzina la chinthu
HWTS-CC001-Kiti Yodziwira HPV16 ndi HPV18 Nucleic Acid (Fluorescence PCR)
Epidemiology
Khansa ya pachibelekero ndi imodzi mwa zotupa zoopsa kwambiri m'njira yoberekera ya akazi. Zawonetsedwa kuti matenda a HPV osatha komanso matenda ambiri ndi chimodzi mwa zifukwa zazikulu za khansa ya pachibelekero. Pakadali pano palibe njira zovomerezeka zochiritsira khansa ya pachibelekero yomwe imayambitsidwa ndi HPV. Chifukwa chake, kuzindikira msanga ndi kupewa matenda a pachibelekero omwe amayambitsidwa ndi HPV ndiye njira zopewera khansa ya pachibelekero. Kukhazikitsa mayeso osavuta, enieni komanso ofulumira ozindikira matenda ndikofunikira kwambiri pakupeza matenda a pachibelekero.
Njira
| Njira | Mtundu |
| FAM | HPV18 |
| VIC/HEX | HPV16 |
| CY5 | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | selo lochotsedwa m'chiberekero |
| Ct | ≤28 |
| CV | ≤10.0% |
| LoD | Makope 500/mL |
| Kufotokozera Mwapadera | Mukagwiritsa ntchito chidachi kuyesa zitsanzo zosakhudzana ndi zomwe zingagwiritsidwe ntchito, zotsatira zake zonse zimakhala zoipa, kuphatikizapo Ureaplasma urealyticum, Chlamydia trachomatis, Candida albicans, Neisseria gonorrhoeae, Trichomonas vaginalis, Mildew, Gardnerella ndi mitundu ina ya HPV yomwe siili mu chidachi. |
| Zida Zogwiritsidwa Ntchito | SLAN®-96P Real-Time PCR SystemsMachitidwe a Biosystems 7500 a Real-Time PCR QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler. |
Kuyenda kwa Ntchito
Njira 1.
Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), iyenera kuchotsedwa motsatira malangizo.
Njira yachiwiri.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006B, HWTS-3006C), iyenera kuchotsedwa motsatira malangizo. Voliyumu ya chitsanzo chochotsedwa ndi 200μL, ndipo voliyumu yovomerezeka ya elution ndi 80µL.
Njira 3.
QIAamp DNA Mini Kit (51304) yopangidwa ndi QIAGEN kapena TIANamp Virus DNA/RNA Kit (YDP315) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd., iyenera kuchotsedwa motsatira malangizo. Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL, ndipo kuchuluka koyenera kwa elution ndi 80µL.
