Kachilombo ka Hepatitis E
Dzina la chinthu
HWTS-HP006 Hepatitis E Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka Hepatitis E (HEV) ndi kachilombo ka RNA komwe kamayambitsa mavuto azaumoyo padziko lonse lapansi. Kali ndi mitundu yosiyanasiyana ya kachilombo ndipo kamatha kufalikira kudzera m'mitundu yosiyanasiyana ya matenda. Ndi chimodzi mwa matenda ofunikira kwambiri a ana ndipo chimayambitsa mavuto aakulu kwa anthu ndi nyama. HEV imafalikira makamaka kudzera m'matenda opatsirana kudzera m'chimbudzi, ndipo imatha kufalikira kudzera m'mazira kapena m'magazi. Pakati pawo, m'njira yofalitsira kudzera m'chimbudzi, madzi ndi chakudya chodetsedwa ndi HEV chimafalikira kwambiri, ndipo chiopsezo cha matenda a HEV mwa anthu ndi nyama n'chokwera [1-2].
Njira
| FAM | HEV nucleic acid |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chitsulo cha pakhosi |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi 500/μL |
| Kufotokozera Mwapadera | Kachilombo ka Hepatitis E (HEV) ndi kachilombo ka RNA komwe kamayambitsa mavuto azaumoyo padziko lonse lapansi. Kali ndi mitundu yosiyanasiyana ya kachilombo ndipo kamatha kufalikira kudzera m'mitundu yosiyanasiyana ya matenda. Ndi chimodzi mwa matenda ofunikira kwambiri a ana ndipo chimayambitsa mavuto aakulu kwa anthu ndi nyama. HEV imafalikira makamaka kudzera m'matenda opatsirana kudzera m'chimbudzi, ndipo imatha kufalikira kudzera m'mazira kapena m'magazi. Pakati pawo, m'njira yofalitsira kudzera m'chimbudzi, madzi ndi chakudya chodetsedwa ndi HEV chimafalikira kwambiri, ndipo chiopsezo cha matenda a HEV mwa anthu ndi nyama n'chokwera [1-2]. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Dongosolo la PCR Logwiritsidwa Ntchito la Applied Biosystems 7500 Fast Real-Time Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Iyenera kuchotsedwa motsatira malangizo. Voliyumu yovomerezeka ya elution ndi 80µL.
Njira yachiwiri
TIANamp Virus DNA/RNA Kit (YDP315-R) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. Iyenera kuchotsedwa motsatira malangizo. Kuchuluka kwa chitsanzo chochotsedwa ndi 140μL. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 60µL.v
