Kuchuluka kwa kachilombo ka Hepatitis B
Dzina la chinthu
HWTS-HP002-Hepatitis B Virus Detection Genotyping Kit (Fluorescent PCR)
Epidemiology
Epidemiology
Njira
| NjiraDzina | Chida Chothandizira 1 | Reaction Buffer 2 |
| FAM | HBV-C | HBV-D |
| VIC/HEX | HBV-B | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ Mumdima |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Seramu, Plasma |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 1 × 102IU/mL |
| Kufotokozera Mwapadera | Palibe njira yolumikizirana pakati pa kachilombo ka hepatitis C, kachilombo ka human cytomegalovirus, kachilombo ka Epstein-Barr, kachilombo ka human immunodeficiency, kachilombo ka hepatitis A, syphilis, kachilombo ka herpes, kachilombo ka influenza A, propionibacterium acnes (PA), ndi zina zotero. |
| Zida Zogwiritsidwa Ntchito | Ikhoza kufanana ndi zida zazikulu za PCR za fluorescent zomwe zili pamsika. Machitidwe a PCR a ABI 7500 Real-Time Machitidwe a PCR a ABI 7500 Ofulumira Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a LightCycler®480 a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo, kuchuluka kwa chitsanzo chochotsedwa ndi200μL, ndipo kuchuluka kwa lution komwe kumalimbikitsidwa ndi80μL.
Chothandizira kuchotsa chomwe chikulangizidwa: Nucleic Acid Extraction kapena Purification Reagent (YDP315). Kuchotsa kuyenera kuchitika motsatira malangizo. Kuchuluka kwa chitsanzo chochotsedwa ndi 200µL, ndipo kuchuluka kwa elution komwe kukulangizidwa ndi 100µL.
