DNA ya Hepatitis B Virus Quantitative Fluorescence
Dzina la chinthu
HWTS-HP015 Hepatitis B Virus DNA Quantitative Fluorescence Diagnostic Kit (Fluorescence PCR)
Epidemiology
Chiwindi cha chiwindi B ndi matenda omwe amayamba chifukwa cha kachilombo ka hepatitis B (HBV), komwe kamadziwika kwambiri ndi zilonda zotupa m'chiwindi, ndipo kangayambitse kuwonongeka kwa ziwalo zingapo. Odwala matenda a chiwindi B amawonetsedwa m'chipatala monga kutopa, kusowa chilakolako cha chakudya, kutupa kwa miyendo kapena ziwalo zina, komanso chiwindi chifukwa cha kulephera kugwira ntchito bwino kwa chiwindi. Anthu asanu pa 100 aliwonse omwe ali ndi kachilomboka ndi 95% ya anthu omwe ali ndi kachilomboka sangathe kuchotsa kachilomboka bwino, zomwe zimapangitsa kuti kachilomboka kasapitirire, ndipo matenda ena osatha pamapeto pake amakhala cirrhosis ya chiwindi ndi khansa ya chiwindi.[1-4].
Njira
| FAM | HBV-DNA |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | seramu yatsopano, Plasma |
| Tt | ≤42 |
| CV | ≤5.0% |
| LoD | 5 IU/mL |
| Kufotokozera Mwapadera | Zotsatira zenizeni zikusonyeza kuti milandu yonse 50 ya zitsanzo za seramu za HBV DNA zabwino ndi zoipa; zotsatira za mayeso obwerezabwereza zikusonyeza kuti palibe kusinthana pakati pa zida izi ndi ma virus ena (HAV, HCV, DFV, HIV) kuti apeze nucleic acid pogwiritsa ntchito zitsanzo za magazi, ndi majini a anthu. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Virus DNA/RNA Kit (HWTS-3017) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.. Kuchotsa kuyenera kuchitika motsatira malangizo, kuchuluka kwa chitsanzo chochotsedwa ndi 300μL, ndipo kuchuluka kwa elution komwe kukulangizidwa ndi 70μL.
