Kachilombo ka Hepatitis A
Dzina la chinthu
HWTS-HP005 Hepatitis A Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Kachilombo ka Hepatitis A (HAV) ndiye chifukwa chachikulu cha matenda a chiwindi a pachimake. Kachilomboka ndi kachilombo ka RNA kokhala ndi positive-sense single-stranded RNA virus ndipo kali m'gulu la Hepadnavirus la banja la Picornaviridae. Kachilombo ka Hepatitis A, komwe kamafalikira makamaka kudzera mu njira ya fecal-mouth, komwe kamalimbana ndi kutentha, ma acid, ndi zinthu zambiri zachilengedwe, kumatha kukhala ndi moyo kwa nthawi yayitali m'madzi a m'nyanja, m'madzi, m'nthaka, kapena m'madzi a m'nyanja [1-3]. Kachilomboka kamafalikira chifukwa chokhala ndi chakudya kapena madzi oipitsidwa, kapena mwachindunji kamafalikira kuchokera kwa munthu ndi munthu. Zakudya zokhudzana ndi HAV ndi monga oyster ndi clams, sitiroberi, raspberries, ma blueberries, madeti, ndiwo zamasamba zobiriwira, ndi tomato wouma pang'ono [4-6].
Njira
| FAM | HAV nucleic acid |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Madzi: miyezi 9, Lyophilized: miyezi 12 |
| Mtundu wa Chitsanzo | Seramu/chopondapo |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makopi awiri/μL |
| Kufotokozera Mwapadera | Gwiritsani ntchito zidazi poyesa mavairasi ena a chiwindi monga hepatitis B, C, D, E, enterovirus 71, kachilombo ka coxsackie, kachilombo ka Epstein-Barr, norovirus, HIV ndi majini a anthu. Palibe njira yodziwira matenda osiyanasiyana. |
| Zida Zogwiritsidwa Ntchito | Dongosolo la PCR la Applied Biosystems 7500 Real-Time Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Dongosolo la LightCycler®480 Real-Time PCR LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Zitsanzo za seramu
Njira 1.
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Iyenera kuchotsedwa motsatira malangizo. Voliyumu yovomerezeka ya elution ndi 80µL.
Njira yachiwiri.
TIANamp Virus DNA/RNA Kit (YDP315-R) yopangidwa ndi Tiangen Biotech (Beijing) Co., Ltd. Iyenera kuchotsedwa motsatira malangizo. Kuchuluka kwa chitsanzo chochotsedwa ndi 140μL. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 60µL.
2.Zitsanzo za Chidendene
Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). Iyenera kuchotsedwa motsatira malangizo. Voliyumu yovomerezeka ya elution ndi 80µL.
