Mitundu 11 ya Tizilombo Toyambitsa Kupuma Youma mu Firiji Nucleic Acid
Dzina la chinthu
HWTS-RT190 -Youma mufiriji Mitundu 11 ya Tizilombo Toyambitsa Kupuma Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda opatsirana m'mapapo ndi matenda ofunikira kwambiri omwe amaika pachiwopsezo thanzi la anthu. Kafukufuku wasonyeza kuti matenda ambiri opatsirana m'mapapo amayamba chifukwa cha mabakiteriya ndi/kapena mavairasi omwe amafalikira kwa wodwala, zomwe zimapangitsa kuti matendawa achuluke kapena kufa. Chifukwa chake, kuzindikira kachilomboka kungapereke chithandizo choyenera ndikuwonjezera kupulumuka kwa wodwalayo [1,2]. Komabe, njira zachikhalidwe zodziwira matenda opatsirana m'mapapo zimaphatikizapo kuyezetsa kwa microscopic, chikhalidwe cha mabakiteriya, ndi kuyezetsa chitetezo chamthupi. Njirazi ndi zovuta, zimatenga nthawi, zimafuna luso laukadaulo, ndipo zimakhala ndi mphamvu zochepa. Kuphatikiza apo, sizingazindikire matenda angapo mu chitsanzo chimodzi, zomwe zimapangitsa kuti zikhale zovuta kupatsa madokotala chidziwitso cholondola chothandizira. Zotsatira zake, mankhwala ambiri akadali mu gawo la mankhwala, zomwe sizimangofulumizitsa kuzungulira kwa kukana kwa mabakiteriya, komanso zimakhudza kuzindikira kwa odwala nthawi yake [3]. Matenda a Common Haemophilus influenzae, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, ndi Legionella pneumophila ndi matenda ofunikira omwe amayambitsa matenda a nosocomial respiratory tract [4,5]. Chida choyeserachi chimazindikira ndikuzindikira ma nucleic acid enieni a matenda omwe ali pamwambapa mwa anthu omwe ali ndi zizindikiro za matenda opumira, ndipo chimachiphatikiza ndi zotsatira zina za labotale kuti zithandize kuzindikira matenda opumira.
Magawo aukadaulo
| Malo Osungirako | 2-30℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chitsulo cha pakhosi |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | LoD ya kit ya Klebsiella pneumoniae ndi 500 CFU/mL; LoD ya Streptococcus pneumoniae ndi 500 CFU/mL; LoD ya Haemophilus influenzae ndi 1000 CFU/mL; LoD ya Pseudomonas aeruginosa ndi 500 CFU/mL; LoD ya Acinetobacter baumannii ndi 500 CFU/mL; LoD ya Stenotrophomonas maltophilia ndi 1000 CFU/mL; LoD ya Bordetella pertussis ndi 500 CFU/mL; LoD ya Bordetella parapertussis ndi 500 CFU/mL; LoD ya Mycoplasma pneumoniae ndi 200 copies/mL; LoD ya Legionella pneumophila ndi 1000 CFU/mL; Mlingo wa matenda a Chlamydia pneumoniae ndi 200 makope/mL. |
| Kufotokozera Mwapadera | Palibe njira yolumikizirana pakati pa kit ndi tizilombo tina tomwe timapezeka m'mapapo kunja kwa malo oyesera, mwachitsanzo Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, ndi Candida tropicalis. |
| Zida Zogwiritsidwa Ntchito | Mtundu Woyamba: Applied Biosystems 7500 Real-Time PCR Systems, Applied Biosystems 7500 Fast Real-Time PCR Systems, QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni, SLAN-96P Machitidwe a PCR a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Real-Time PCR Systems, LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, ukadaulo wa Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System. Mtundu Wachiwiri: EudemonTMAIO800 (HWTS-EQ007) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Kuyenda kwa Ntchito
Mtundu Woyamba: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ndi omwe akulimbikitsidwa kuti achotsedwe ndipo njira zotsatirazi ziyenera kuchitidwa motsatira IFU ya Kit.
