Enterovirus Universal, EV71 ndi CoxA16
Dzina la Chinthu
HWTS-EV026B-Enterovirus Universal, EV71 ndi CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
HWTS-EV020Y/Z-Chida chodziwira Enterovirus Universal, EV71 ndi CoxA16 Nucleic Acid (Fluorescence PCR) chouma ndi kuzizira
Satifiketi
CE/MDA（HWTS-EV026）
Epidemiology
Matenda a mkamwa mwa manja (HFMD) ndi matenda opatsirana ofala kwambiri mwa ana. Amapezeka kwambiri mwa ana osakwana zaka 5, ndipo angayambitse nsungu m'manja, mapazi, pakamwa ndi ziwalo zina, ndipo ana ochepa angayambitse mavuto monga myocarditis, pulmonary edema, aseptic meningoencephalitis, ndi zina zotero. Ana omwe ali ndi matenda akuluakulu amakula mofulumira ndipo amatha kufa ngati salandira chithandizo mwachangu.
Pakadali pano, ma serotype 108 a ma enterovirus apezeka, omwe amagawidwa m'magulu anayi: A, B, C ndi D. Ma Enterovirus omwe amayambitsa HFMD ndi osiyanasiyana, koma enterovirus 71 (EV71) ndi coxsackievirus A16 (CoxA16) ndi omwe amapezeka kwambiri ndipo kuwonjezera pa HFMD, angayambitse mavuto akulu apakati pa mitsempha monga meningitis, encephalitis, ndi acute flaccid paralysis.
Njira
| FAM | Kachilombo ka Enterovirus |
| VIC (HEX) | CoxA16 |
| ROX | EV71 |
| CY5 | Kulamulira kwamkati |
Magawo aukadaulo
| Malo Osungirako | Madzi: ≤-18℃ MumdimaKusungunuka kwa mpweya (Lyophilization): ≤30℃ |
| Nthawi yokhalitsa | Madzi: miyezi 9Kuchepetsa mpweya m'thupi: miyezi 12 |
| Mtundu wa Chitsanzo | Chitsanzo cha swab ya pakhosi, madzi a Herpes |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | Makopi 500/mL |
| Zida Zogwiritsidwa Ntchito | Ikhoza kufanana ndi zida zazikulu za PCR za fluorescent zomwe zili pamsika.Machitidwe a PCR a ABI 7500 Real-Time Machitidwe a PCR a ABI 7500 Ofulumira Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Makina a PCR a LightCycler®480 a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Yankho Lonse la PCR
Chotsukira chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo. Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL, ndipo kuchuluka kwa elution komwe kukulangizidwa ndi 80μL.
Chotsukira chomwe chikulangizidwa: Chotsukira cha Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kuchotsa kuyenera kuchitika motsatira malangizo. Zitsanzo zotsukira ndi oropharyngeal swabs kapena zitsanzo zamadzimadzi a herpes a odwala omwe adasonkhanitsidwa pamalopo. Onjezani swabs zomwe zasonkhanitsidwa mwachindunji ku Macro & Micro-Test Sample Release Reagent, vortex ndikusakaniza bwino, ikani kutentha kwa chipinda kwa mphindi 5, tulutsani kenako tembenuzani ndikusakaniza bwino kuti mupeze RNA ya chitsanzo chilichonse.
Chothandizira kuchotsa chomwe chikulangizidwa: QIAamp Viral RNA Mini Kit (52904) ndi QIAGEN kapena Nucleic Acid Extraction kapena Purification Reagent (YDP315-R). Kuchotsa kuyenera kuchitika motsatira malangizo a buku.
