Enterovirus Universal, EV71 ndi CoxA16
Dzina lazogulitsa
HWTS-EV026B-Enterovirus Universal, EV71 ndi CoxA16 Nucleic Acid Detection Kit(Fluorescence PCR)
HWTS-EV020Y/Z-Fluorescence PCR, EV71 ndi CoxA16 Nucleic Acid Detection Kit (Fluorescence PCR)
Satifiketi
CE/MDA (HWTS-EV026)
Epidemiology
Matenda a Hand-foot-mouth (HFMD) ndi matenda opatsirana omwe amapezeka mwa ana. Nthawi zambiri zimachitika kwa ana osakwana zaka 5, ndipo zingayambitse nsungu pamanja, mapazi, pakamwa ndi mbali zina, ndi ochepa ana angayambitse mavuto monga myocarditis, m`mapapo mwanga edema, aseptic meningoencephalitis, etc. Ana omwe ali ndi matenda aakulu amawonongeka mofulumira ndipo amatha kufa ngati sanalandire chithandizo mwamsanga.
Panopa, 108 serotypes a enteroviruses apezeka, amene anawagawa m'magulu anayi: A, B, C ndi D. Enteroviruses amene amachititsa HFMD zosiyanasiyana, koma enterovirus 71 (EV71) ndi coxsackievirus A16 (CoxA16) ndi ambiri komanso kuwonjezera HFMD, zingachititse kwambiri chapakati mantha dongosolo encephalitis, ndi paralysis .
Channel
| FAM | Matenda a Enterovirus |
| VIC (HEX) | KoxA16 |
| Mtengo ROX | EV71 |
| CY5 | Ulamuliro wamkati |
Technical Parameters
| Kusungirako | Zamadzimadzi: ≤-18 ℃ MumdimaLyophilization: ≤30 ℃ |
| Alumali moyo | Madzi: miyezi 9Lyophilization: miyezi 12 |
| Mtundu wa Chitsanzo | Sampuli ya pakhosi, Herpes fluid |
| Ct | ≤38 |
| CV | ≤5.0％ |
| LoD | 500 Makopi / ml |
| Zida Zogwiritsira Ntchito | Itha kufanana ndi zida za fulorosenti za PCR pamsika.ABI 7500 Real-Time PCR Systems ABI 7500 Fast Real-Time PCR Systems SLAN-96P Real-Time PCR Systems QuantStudio®5 Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Total PCR Solution
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Voliyumu yotulutsidwa ndi 200μL, ndipo voliyumu yovomerezeka ndi 80μL.
Zopangira zopangira zovomerezeka: Macro & Micro-Test Sample Release Reagent (HWTS-3005-8). Kuchotsa kuyenera kuchitidwa molingana ndi bukhu la malangizo. Zitsanzo za m'zigawo ndi oropharyngeal swabs kapena herpes madzimadzi zitsanzo za odwala amene anasonkhanitsa pa malo. Onjezani ma swabs osonkhanitsidwa mwachindunji ku Macro & Micro-Test Sample Release Reagent, vortex ndikusakaniza bwino, ikani kutentha kwa mphindi 5, chotsani ndikutembenuza ndikusakaniza bwino kuti mupeze RNA yachitsanzo chilichonse.
Regent yovomerezeka yochotsa: QIAamp Viral RNA Mini Kit (52904) yolembedwa ndi QIAGEN kapena Nucleic Acid Extraction kapena Purification Reagent (YDP315-R). M'zigawozi zichitike motsatira ndondomeko ya malangizo.
