Endoruvers Universal, Ev71 ndi Coxa16
Dzina lazogulitsa
HWTS-Ev026b-Endorurrus Universal, Ev71 ndi Cox71 ndi Coxa16 Nyukiliya a Acid Couctic Clay Kit (Fluorescence PCR)
Hwts-ev020y / z-Kuwuma chipongwe enkovis eyiti, EV71 ndi Cox71 ndi Coxa16 Nuxa
Chiphaso
CE / MDA (HWTS-Ev026)
Epidemiology
Matenda amkamwa a m'manja (HFMD) ndiofala pachimake matenda mwa ana. Zimachitika kwambiri mwa ana osakwana zaka 5, ndipo zimatha kuyambitsa herper m'manja, mapazi, kamwa ndi mbali zina, ndipo ana a mulc. ana ali ndi vuto lalikulu Nkhumba zimawonongeka mwachangu ndipo zimayamba kuphedwa ngati sizikuthandizika mwachangu.
Pakadali pano, ma setro 108 amapezeka, omwe amagawidwa m'magulu anayi: a, c ndi d.) kuwonjezera pa HFMD, kungayambitse zovuta zazikuluzikulu zamanjenje monga meningitis, encephalitis, ndi pachimake flaccid ziwalo.
Ngalande
Banja | Lowa m'malo |
Vic (Hex) | Coxa16 |
Rox | EV71 |
Cy5 | Kuwongolera kwamkati |
Magawo aluso
Kusunga | Madzi: ≤-18 ℃ mdimaLyophillization: ≤30 ℃ |
Alumali-Moyo | Madzi: miyezi 9Lyophillization: miyezi 12 |
Mtundu wofanana | Throat swab sample, herpes madzimadzi |
Ct | ≤38 |
CV | ≤5.0% |
Lodu | 500copees / ml |
Zida Zogwirizira | Itha kufanana ndi zida zazikulu za PCR pamsika.ABI 7500 PCR Systems Abi 7500 mwachangu pcr pcr systems Slan-96P yeniyeni PCR Systems Ma pcr-5 a PCR Chiwonetsero cha Specycler®480 PCR Systems Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Chiwerengero cha PCR
Kulimbikitsidwa komwe kumalimbikitsa: Macro & Micro-Chef CerAr DNA / RNTS-3017) (zomwe zingagwiritsidwe ntchito ndi ma macro-eq011) Co., Ltd. Kuchotsa kuyenera kuchitika malinga ndi buku la Prince. Voliyumu yoyendetsedwa ndi 200μc, ndipo kuchuluka kwa Evest Evel ndi 80μl.
Cholinga cholimbikitsidwa: Macro & Macro-Chemip Stupple (HWTS-3005-8). Kuchotsa kuyenera kuchitika molingana ndi buku la Prince. Zitsanzo zowonjezera ndi orpharyngeal swabs kapena zitsanzo zamadzimadzi za odwala omwe adasonkhanitsa pamalopo. Onjezani ma swab omwe anasonkhanitsidwa mwachindunji ku Macro & Chemex Temple Regint, Vortex ndikusakaniza bwino, ikani kutentha kwa mphindi 5, kutuluka kenako nkusakaniza ndikusakaniza.
Kulimbikitsidwa Kutulutsa: Qaamp Virul Rna Kit Kit (52904) ndi QDP315-R). Kuchotsa kuyenera kuchitika molingana ndi buku la malangizo.