Kachilombo ka Enterovirus 71 (EV71)
Dzina la chinthu
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a mkamwa mwa manja ndi matenda opatsirana omwe amayamba chifukwa cha ma enterovirus (EV). Pakadali pano, mitundu 108 ya ma enterovirus yapezeka, yomwe yagawidwa m'magulu anayi: A, B, C ndi D. Pakati pawo, enterovirus EV71 ndi CoxA16 ndi omwe amayambitsa matenda akuluakulu. Matendawa amapezeka kwambiri mwa ana osakwana zaka 5, ndipo angayambitse mabala m'manja, mapazi, pakamwa ndi ziwalo zina. Ana ochepa amakhala ndi mavuto monga myocarditis, pulmonary edema, ndi aseptic meningoencephalitis.
Njira
| FAM | EV71 |
| ROX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 9 |
| Mtundu wa Chitsanzo | Chimbudzi cha m'kamwa,Madzi a Herpes |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | Makopi 500/mL |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Machitidwe 480 a PCR a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Chotsukira chomwe chikulangizidwa: Chotsukira cha Macro & Micro-Test Sample Release Reagent (HWTS-3005-8), ndipo chotsukiracho chiyenera kuchitika motsatira malangizo ogwiritsira ntchito. Zitsanzo zomwe zachotsedwa ndi oropharyngeal swabs kapena zitsanzo zamadzimadzi a herpes kuchokera kwa odwala omwe adatengedwa pamalopo. Onjezani swabs zomwe zasonkhanitsidwa mu Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) mwachindunji, sakanizani bwino, ikani kutentha kwa chipinda kwa mphindi 5, zitulutseni kenako mutembenuzire kuti zisakanize bwino, kuti mupeze RNA ya chitsanzo chilichonse.
Njira yachiwiri.
Reagent yochotsera yomwe ikulangizidwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ndipo kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Voliyumu ya chitsanzo chochotsedwa ndi 200µL, ndipo voliyumu yovomerezeka ya elution ndi 80µL.
Njira 3.
Chothandizira kuchotsa chomwe chikuyembekezeredwa: QIAamp Viral RNA Mini Kit (52904) ndi QIAGEN kapena TIANamp Virus DNA/RNA Kit (YDP315-R), ndipo kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa chitsanzo chochotsedwa ndi 140μL, ndipo kuchuluka kwa elution komwe kukuyembekezeredwa ndi 60μL.
