Enterovirus 71 (EV71)
Dzina la malonda
HWTS-EV003- Enterovirus 71 (EV71) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda a Hand-foot-mouth ndi matenda opatsirana omwe amayamba chifukwa cha enteroviruses (EV).Pakalipano, mitundu 108 ya serotypes ya enteroviruses yapezeka, yomwe imagawidwa m'magulu anayi: A, B, C ndi D. Pakati pawo, enterovirus EV71 ndi CoxA16 ndizo tizilombo toyambitsa matenda.Matendawa nthawi zambiri amapezeka mwa ana osakwana zaka 5, ndipo angayambitse nsungu pamanja, mapazi, pakamwa ndi mbali zina.Ana ochepa amatha kukhala ndi zovuta monga myocarditis, pulmonary edema, ndi aseptic meningoencephalitis.
Channel
| FAM | EV71 |
| Mtengo ROX | Ulamuliro Wamkati |
Magawo aukadaulo
| Kusungirako | ≤-18 ℃ |
| Alumali moyo | 9 miyezi |
| Mtundu wa Chitsanzo | Oropharyngeal swab,Herpes madzimadzi |
| Ct | ≤35 |
| CV | <5.0% |
| LoD | 500 Makopi / ml |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR Systems LineGene 9600 Plus Real-Time PCR Detection Systems MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Njira 1.
Analimbikitsa m'zigawo reagent: Macro & yaying'ono-Test Chitsanzo Release Reagent (HWTS-3005-8), ndi m'zigawo ayenera kuchitidwa molingana ndi malangizo ntchito.Zitsanzo zomwe zatulutsidwa ndi oropharyngeal swabs kapena zitsanzo za herpes zamadzimadzi kuchokera kwa odwala omwe adatolera pamalopo.Onjezani ma swabs omwe asonkhanitsidwa mu Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) mwachindunji, vortex ndikusakaniza bwino, ikani kutentha kwa mphindi 5, kuwatulutsa ndikutembenuza kuti musakanize bwino, kuti mupeze RNA. wa chitsanzo chilichonse.
Njira 2.
Zomwe zimalangizidwa m'zigawo: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., ndipo kuchotsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito.Voliyumu yotulutsidwa ndi 200µL, ndipo voliyumu yovomerezeka ndi 80µL.
Njira 3.
Zopangira zopangira zovomerezeka: QIAamp Viral RNA Mini Kit (52904) yolembedwa ndi QIAGEN kapena TIANamp Virus DNA/RNA Kit (YDP315-R), ndipo kuchotsako kuyenera kuchitidwa motsatira malangizo ogwiritsira ntchito.Voliyumu yotulutsidwa ndi 140μL, ndipo voliyumu yovomerezeka ndi 60µL.
