Mitundu Isanu ndi Itatu ya Mavairasi Opumira
Dzina la chinthu
HWTS-RT184-Mitundu Isanu ndi Itatu ya Mavairasi Opumira Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Matenda opatsirana m'mapapo ndi mtundu wofala kwambiri wa matenda a anthu, omwe angachitike mwa amuna kapena akazi, zaka ndi dera lililonse, ndipo ndi chimodzi mwa zifukwa zazikulu zoyambitsa matenda ndi imfa padziko lonse lapansi.[1]Matenda opatsirana omwe amapezeka m'mapapo ndi monga kachilombo ka fuluwenza A (IFV A), kachilombo ka fuluwenza B (IFV B), kachilombo ka respiratory syncytial, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) ndi mycoplasma pneumoniae, ndi zina zotero.[2,3]Zizindikiro ndi zizindikiro za matenda opatsirana m'mapapo zimafanana, koma matenda opatsirana ndi tizilombo toyambitsa matenda osiyanasiyana ali ndi njira zosiyanasiyana zochiritsira, zotsatira zake zochiritsira komanso momwe matendawo amayambira.[4,5]Pakadali pano, njira zazikulu zodziwira matenda opatsirana pogonana m'ma laboratories ndi izi: kudzipatula kwa kachilombo, kuzindikira ma antigen ndi kuzindikira nucleic acid. Kit iyi imazindikira ndikuzindikira ma virus nucleic acid mwa anthu omwe ali ndi zizindikiro za matenda opatsirana pogonana, limodzi ndi zotsatira zina zachipatala komanso za labotale kuti zithandize kuzindikira matenda opatsirana pogonana m'mapiritsi.
Magawo aukadaulo
| Malo Osungirako | 2-8℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Nsalu ya m'mphuno; Nsalu ya m'mphuno |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Makope 200/mL |
| Kufotokozera Mwapadera | Kuyanjana kwa kachilomboka: Palibe kugwirizana pakati pa kachilomboka ndi kachilombo ka Boca, Cytomegalovirus, kachilombo ka Epstein-Barr, kachilombo ka Herpes simplex, kachilombo ka varicella zoster, kachilombo ka Mumps, kachilombo ka Enterovirus, kachilombo ka chikuku, kachilombo ka coronavirus ka anthu, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, DNA ya genomic ya anthu. Mayeso oletsa kusokoneza: Sankhani mucin (60mg/mL), magazi a munthu (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride yokhala ndi 5% yotetezera (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Zotsatira zake zasonyeza kuti zinthu zosokoneza zomwe zili pamwambazi |
| Zida Zogwiritsidwa Ntchito | Yogwiritsidwa ntchito pa reagent yozindikira mtundu wa I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Machitidwe a PCR a Nthawi Yeniyeni, SLAN-96P Machitidwe a PCR a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Machitidwe Ozindikira PCR a Nthawi Yeniyeni (FQD-96A, ukadaulo wa Hangzhou Bioer), MA-6000 Wowerengera Kutentha kwa Nthawi Yeniyeni (Suzhou Molarray Co., Ltd.), BioRad CFX96 Machitidwe a PCR a Nthawi Yeniyeni, BioRad CFX Opus 96 Machitidwe a PCR a Nthawi Yeniyeni. Yogwiritsidwa ntchito pa reagent yozindikira mtundu wa II: EudemonTM AIO800 (HWTS-EQ007) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Kuyenda kwa Ntchito
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), ndi Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (yomwe ingagwiritsidwe ntchito ndi EudemonTM AIO800 (HWTS-EQ007)) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Kuchuluka kwa chitsanzo chochotsedwa ndi 200μL ndipo kuchuluka kwa elution komwe kumalimbikitsidwa ndi 150μL.
