Borrelia Burgdorferi Nucleic Acid
Dzina la chinthu
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Matenda a Lyme amayamba chifukwa cha matenda a Borrelia burgdorferi ndipo amafalikira makamaka pakati pa nyama zomwe zimakhalamo, pakati pa nyama zomwe zimakhalamo ndi anthu ndi nkhupakupa zolimba. Borrelia burgdorferi ingayambitse matenda a Erythema chronicum migrans a anthu, komanso matenda okhudzana ndi machitidwe osiyanasiyana monga mtima, mitsempha, ndi mafupa, ndi zina zotero, ndipo zizindikiro zake zimakhala zosiyanasiyana. Malinga ndi kukula kwa matenda, amatha kugawidwa m'magulu awiri: matenda oyamba kumene, matenda opatsirana pakati komanso matenda opitirira muyeso, omwe amawononga thanzi la anthu. Chifukwa chake, pozindikira matenda a Borrelia burgdorferi, ndikofunikira kwambiri kukhazikitsa njira yosavuta, yeniyeni komanso yachangu yodziwira matenda a Borrelia burgdorferi.
Njira
| FAM | DNA ya Borrelia burgdorferi |
| VIC/HEX | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Kuyesa magazi athunthu |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | Makope 500/mL |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a ABI 7500 Real-Time Machitidwe a PCR a ABI 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Kiti ya Midi ya Magazi ya QIAamp DNA yolembedwa ndi Qiagen (51185).It ziyenera kuchotsedwamotsatira malamulo onseku malangizo, ndipo voliyumu yovomerezeka ya elution ndi100μL.
Njira yachiwiri.
MagaziGDNA ya enomicEzida zochotsera (DP318,Ayi.: JingchangMbiri ya Chipangizo20210062) yopangidwa ndi Tiangen Biochemical Technology (Beijing) Co., Ltd.. It ziyenera kuchotsedwamotsatira malamulo onseku malangizo, ndipo voliyumu yovomerezeka ya elution ndi100μL.
Njira 3.
Kiti Yoyeretsera DNA ya Wizard® Genomic (A1120) yopangidwa ndi Promega.It ziyenera kuchotsedwamotsatira malamulo onseku malangizo, ndipo voliyumu yovomerezeka ya elution ndi100μL.
