Mitundu 28 ya Kachilombo ka Papilloma Virus (16/18 Typing) Nucleic Acid
Dzina la malonda
HWTS-CC006A-28 Mitundu ya Kachilombo ka Papilloma Virus (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Khansara ya khomo pachibelekeropo ndi imodzi mwa zotupa zowopsa za chiberekero cha ubereki. Kafukufuku wasonyeza kuti matenda a HPV osalekeza komanso matenda angapo ndi chimodzi mwazomwe zimayambitsa khansa ya pachibelekero. Pakadali pano, chithandizo chodziwika bwino cha khansa ya khomo pachibelekero choyambitsidwa ndi HPV sichinapezekebe, kotero kupeza msanga komanso kupewa matenda a khomo pachibelekero chifukwa cha HPV ndiye chinsinsi chopewera khansa ya pachibelekero. Ndikofunikira kwambiri kukhazikitsa mayeso osavuta, enieni komanso ofulumira a etiology kuti adziwe matenda ndi chithandizo cha khansa ya pachibelekero.
Channel
| Reaction Mix | Channel | Mtundu |
| PCR-Mix1 | FAM | 18 |
| VIC (HEX) | 16 | |
| Mtengo ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Ulamuliro Wamkati | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC (HEX) | 26, 44, 61, 81 | |
| Mtengo ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Ulamuliro Wamkati |
Technical Parameters
| Kusungirako | Madzi: ≤-18 ℃ |
| Alumali moyo | 12 miyezi |
| Mtundu wa Chitsanzo | Cervical Swab, Vaginal Swab, Mkodzo |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | 300 Makopi / ml |
| Zida Zogwiritsira Ntchito | Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 Fast Real-Time PCR Systems Zithunzi za QuantStudio®5 Real-Time PCR Systems SLAN-96P Real-Time PCR Systems LightCycler®480 Real-Time PCR system LineGene 9600 Plus Real-Time PCR Detection System MA-6000 Real-Time Quantitative Thermal Cycler BioRad CFX96 Real-Time PCR System BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda Ntchito
Analimbikitsa m'zigawo reagent: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid (CTS) Extra3006W HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu sitepe 2.1, ndiyeno m'zigawozi uyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito pochotsa izi. Voliyumu yovomerezeka ndi 80μL.
Zopangira zopangira zovomerezeka: QIAamp DNA Mini Kit (51304) kapena Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu sitepe 2.1, ndiyeno m'zigawozi uyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito pochotsa izi. Voliyumu yotulutsidwa ya zitsanzo zonse ndi 200μL, ndipo voliyumu yovomerezeka ndi 100μL.
