Mitundu 28 ya ma virus oopsa a papilloma (16/18 kulembera) nucleic acid
Dzina lazogulitsa
Mitundu ya HWTS-CC006A ya virus yoopsa ya Papilloma (16/18 Kulemba) Nyukiliya ya Asic Nucleic Acid Kit (Fluorescence PCR)
Epidemiology
Khansa yazenera ndi imodzi mwazotupa zoyipa kwambiri za kubereka kwa akazi. Kafukufuku wasonyeza kuti matenda a HPV amalimbikira ndi matenda angapo ndi amodzi mwa zifukwa zazikulu za khansa ya khosi. Pakadali pano, chithandizo chamankhwala chodziwika bwino chikuchepa cha khansa yoyambitsidwa ndi HPV, motero kupezeka koyambirira komanso kupewa matenda opatsirana pazenera. Ndikofunikira kwambiri kukhazikitsa chiyeso chosadziwika, chodziwika bwino komanso chofufuzira chakumwa matenda ndi chithandizo cha khansa ya khomo.
Ngalande
Kusakaniza | Ngalande | Mtundu |
PCR-Dint1 | Banja | 18 |
Vic (Hex) | 16 | |
Rox | 31, 33, 35, 39, 39, 45, 51, 56, 58, 58, 66, 66, 68, 68 | |
Cy5 | Kuwongolera kwamkati | |
PCR-CAST2 | Banja | 6, 11, 54, 83 |
Vic (Hex) | 26, 44, 61, 81 | |
Rox | 40, 42, 43, 53, 73, 82 | |
Cy5 | Kuwongolera kwamkati |
Magawo aluso
Kusunga | Madzi: ≤-18 ℃ |
Alumali-Moyo | Miyezi 12 |
Mtundu wofanana | Swab yazitsulo, swab, mkodzo |
Ct | ≤28 |
CV | <5.0% |
Lodu | 300Copees / ml |
Zida Zogwirizira | Imagwiritsa ntchito ma biosyystems 7500 pcr PCR Systems Imagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Makupala®5 pcr yell pcr Slan-96P yeniyeni PCR Systems Kuwala®480 pcr pcr system Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Kulimbikitsidwa Kwambiri: Macro & Bigro-Chefre DNA / RNA-3017-50, HWTS-3017-9, 3017-96) (yomwe ingayesedwe mayeso Zovala za Nucleut Acid & Micro-Tech Coun-Tech Co., ltd. Buku lolimbikitsidwa ndi 80μl.
Kulimbikitsidwa Kutulutsa: Qiamp DNA Mini Kit (51304) kapena macro-micro-mayeso oyeserera a Macro & RNA (HWTTS-3020-50). Onjezani milidi ya 200μl kuti muchepetse pellet mu Gawo 2.1, kenako kuchotsera kuyenera kuchitidwa malinga ndi malangizo omwe amagwiritsidwa ntchito pochotsera ichi. Zitsanzo zowonjezereka za 200μc, ndi kuchuluka kwa Evest Evel ndi 100μL.