Mitundu 28 ya Kachilombo ka Papilloma ka Munthu Koopsa Kwambiri (16/18 Type) Nucleic Acid
Dzina la chinthu
HWTS-CC006A-28 Mitundu ya Kachilombo ka Papilloma ka Munthu Koopsa Kwambiri (16/18 Typing) Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Khansa ya pachibelekero ndi imodzi mwa zotupa zoopsa kwambiri m'njira yoberekera ya akazi. Kafukufuku wasonyeza kuti matenda opatsirana a HPV osatha komanso matenda ambiri ndi chimodzi mwa zifukwa zazikulu zomwe zimayambitsa khansa ya pachibelekero. Pakadali pano, mankhwala othandiza omwe amadziwika kuti ndi othandiza akadalibe chifukwa cha khansa ya pachibelekero yomwe imayambitsidwa ndi HPV, kotero kupeza msanga ndi kupewa matenda a pachibelekero omwe amayambitsidwa ndi HPV ndiye chinsinsi chopewera khansa ya pachibelekero. Ndikofunikira kwambiri kukhazikitsa mayeso osavuta, enieni komanso ofulumira ozindikira matenda a khansa ya pachibelekero.
Njira
| Kusakaniza kwa Zokambirana | Njira | Mtundu |
| PCR-Mix1 | FAM | 18 |
| VIC(HEX) | 16 | |
| ROX | 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 | |
| CY5 | Kulamulira Kwamkati | |
| PCR-Mix2 | FAM | 6, 11, 54, 83 |
| VIC(HEX) | 26, 44, 61, 81 | |
| ROX | 40, 42, 43, 53, 73, 82 | |
| CY5 | Kulamulira Kwamkati |
Magawo aukadaulo
| Malo Osungirako | Madzi: ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chisalu cha m'chiberekero, Chisalu cha m'chiberekero, Mkodzo |
| Ct | ≤28 |
| CV | <5.0% |
| LoD | Makope 300/mL |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Chothandizira kuchotsa chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (chomwe chingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu gawo 2.1, kenako kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chothandizira ichi chochotsera. Kuchuluka koyenera kwa elution ndi 80μL.
Chotsukira chomwe chikufunidwa: QIAamp DNA Mini Kit (51304) kapena Macro & Micro-Test Viral DNA/RNA Column (HWTS-3020-50). Onjezani 200μL ya saline wamba kuti muyimitsenso pellet mu gawo 2.1, kenako kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito chotsukira ichi. Kuchuluka kwa zitsanzo zomwe zachotsedwa ndi 200μL, ndipo kuchuluka kwa zotsukira komwe kukufunidwa ndi 100μL.
