Mitundu 18 ya Nucleic Acid ya Kachilombo ka Papilloma ka Munthu Koopsa Kwambiri
Dzina la chinthu
Mitundu ya HWTS-CC018B-18 ya Kiti Yodziwira Matenda a Papilloma Virus ya Anthu Yoopsa Kwambiri (Fluorescence PCR)
Satifiketi
CE
Epidemiology
Khansa ya pachibelekero ndi imodzi mwa zotupa zoopsa kwambiri m'njira yoberekera ya akazi. Kafukufuku wasonyeza kuti matenda osatha komanso matenda ambiri a papillomavirus ya anthu ndi chimodzi mwa zifukwa zazikulu zomwe zimayambitsa khansa ya pachibelekero.
Matenda a HPV m'njira yoberekera ndi ofala pakati pa akazi omwe ali ndi moyo wogonana. Malinga ndi ziwerengero, akazi 70% mpaka 80% akhoza kukhala ndi kachilombo ka HPV kamodzi kokha m'moyo wawo, koma matenda ambiri amadziletsa okha, ndipo akazi oposa 90% omwe ali ndi kachilomboka amakhala ndi chitetezo chamthupi chomwe chingathetse matendawa pakati pa miyezi 6 ndi 24 popanda chithandizo chamankhwala cha nthawi yayitali. Matenda a HPV omwe amakhala pachiwopsezo chachikulu ndiye chifukwa chachikulu cha khansa ya m'chiberekero ndi khansa ya m'chiberekero.
Zotsatira za kafukufuku wapadziko lonse lapansi zasonyeza kuti kupezeka kwa DNA ya HPV yomwe ili pachiwopsezo chachikulu kunapezeka mwa 99.7% ya odwala khansa ya pachibelekero. Chifukwa chake, kuzindikira msanga ndi kupewa HPV ya pachibelekero ndiye chinsinsi choletsa khansa. Kukhazikitsa njira yosavuta, yeniyeni komanso yofulumira yodziwira matenda ndikofunikira kwambiri pakupeza matenda a khansa ya pachibelekero.
Njira
| FAM | HPV 18 |
| VIC (HEX) | HPV 16 |
| ROX | HPV 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 82 |
| CY5 | Kulamulira kwamkati |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ mumdima |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chisalu cha m'chiberekero, Chisalu cha m'chiberekero, Mkodzo |
| Ct | ≤28 |
| CV | ≤5.0％ |
| LoD | Makope 300/mL |
| Kufotokozera Mwapadera | (1) Zinthu Zosokoneza Gwiritsani ntchito zidazi kuti muyese zinthu zotsatirazi zosokoneza, zotsatira zake zonse ndi zoipa: hemoglobin, maselo oyera amagazi, ntchofu ya pachibelekero, metronidazole, lotion ya Jieryin, lotion ya Fuyanjie, mafuta odzola a anthu.(2) Kusinthasintha kwa zochitika Gwiritsani ntchito zidazi kuti muyesere tizilombo toyambitsa matenda tomwe timagwirizana ndi njira zoberekera komanso DNA ya majini ya anthu yomwe ingakhale ndi reactivity yolumikizana ndi zidazi, zotsatira zake zonse ndi zoipa: Zitsanzo za HPV6 zabwino, zitsanzo za HPV11 zabwino, zitsanzo za HPV40 zabwino, zitsanzo za HPV42 zabwino, zitsanzo za HPV43 zabwino, zitsanzo za HPV44 zabwino, zitsanzo za HPV54 zabwino, zitsanzo za HPV67 zabwino, zitsanzo za HPV69 zabwino, zitsanzo za HPV70 zabwino, zitsanzo za HPV71 zabwino, zitsanzo za HPV72 zabwino, zitsanzo za HPV81 zabwino, zitsanzo za HPV83 zabwino, herpes simplex virus type Ⅱ, treponema pallidum, ureaplasma urealyticum, mycoplasma hominis, candida albicans, neisseria gonorrhoeae, trichomonas vaginalis, chlamydia trachomatis ndi human genomic DNA |
| Zida Zogwiritsidwa Ntchito | Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Machitidwe 480 a PCR a Nthawi Yeniyeni Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Yankho Lonse la PCR
Njira 1.
1. Kusankha zitsanzo
2. Kuchotsa asidi wa nyukiliya
3. Onjezani zitsanzo ku makina
Njira yachiwiri.
1. Kusankha zitsanzo
2. Yopanda kuchotsa
3. Onjezani zitsanzo ku makina
