Mitundu 15 ya Gene mRNA ya Papillomavirus ya Anthu Yoopsa Kwambiri E6/E7

Mitundu 15 ya kachilombo ka papilloma ka munthu komwe kali pachiwopsezo chachikulu E6/E7 Gene mRNA Chithunzi Chowonetsedwa

Kufotokozera Kwachidule:

Chida ichi cholinga chake ndi kuzindikira bwino kuchuluka kwa majini 15 a papillomavirus (HPV) E6/E7 mRNA m'maselo otulutsidwa m'chiberekero cha mkazi.

Tumizani imelo kwa ife

Tsatanetsatane wa Zamalonda

Ma tag a Zamalonda

Dzina la chinthu

Mitundu ya HWTS-CC005A-15 ya Human Papillomavirus E6/E7 Gene mRNA Detection Kit (Fluorescence PCR) yomwe ili pachiwopsezo chachikulu

Epidemiology

Khansa ya pachibelekero ndi imodzi mwa mitundu yofala kwambiri ya khansa ya akazi padziko lonse lapansi, ndipo kupezeka kwake kumagwirizana kwambiri ndi ma papillomavirus a anthu (HPV), koma gawo laling'ono chabe la matenda a HPV lomwe limatha kukhala khansa. HPV yomwe ili pachiwopsezo chachikulu imakhudza maselo a epithelial a pachibelekero ndipo imapanga ma oncoprotein awiri, E6 ndi E7. Puloteni iyi imatha kukhudza mapuloteni osiyanasiyana a m'maselo (monga mapuloteni oletsa chotupa pRB ndi p53), kutalikitsa nthawi ya maselo, kukhudza kapangidwe ka DNA ndi kukhazikika kwa majini, ndikusokoneza mayankho a chitetezo cha mthupi oletsa mavairasi ndi chotupa.

Njira

Njira	Chigawo	Kuyesedwa kwa mtundu wa majini
FAM	HPV Reaction Buffer 1	HPV16, 31, 33, 35, 51, 52, 58
VIC/HEX	HPV Reaction Buffer 1	Jini la β-actin la munthu
FAM	HPV Reaction Buffer 2	HPV 18, 39, 45, 53, 56, 59, 66, 68
VIC/HEX	HPV Reaction Buffer 2	Jini la INS la munthu

Magawo aukadaulo

Malo Osungirako	Madzi: ≤-18℃
Nthawi yokhalitsa	Miyezi 9
Mtundu wa Chitsanzo	Nsalu ya pachibelekero
Ct	≤38
CV	<5.0%
LoD	Makope 500/mL
Zida Zogwiritsidwa Ntchito	Dongosolo la PCR la Applied Biosystems 7500 Real-TimeMachitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni Makina a PCR a QuantStudio®5 a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Dongosolo la LightCycler®480 Real-Time PCR Dongosolo Lozindikira la LineGene 9600 Plus Real-Time PCR MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System

Kuyenda kwa Ntchito

Chotsukira chomwe chikulangizidwa: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3020-50-HPV15) chopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito. Kuchuluka kwa kuyeretsa komwe kukulangizidwa ndi 50μL. Ngati chitsanzocho sichinagayidwe mokwanira, chibwezereni ku gawo lachinayi kuti chigayidwenso. Kenako yesani motsatira malangizo ogwiritsira ntchito.

Chothandizira kuchotsa chomwe chikulangizidwa: RNAprep Pure Animal Tissue Total RNA Extraction Kit (DP431). Kuchotsa kuyenera kuchitika motsatira malangizo ogwiritsira ntchito mosamala (Mu gawo lachisanu, onjezerani kawiri kuchuluka kwa yankho logwira ntchito la DNaseI, ndiko kuti, tengani 20μL ya yankho la RNase-Free DNaseI (1500U) mu chubu chatsopano cha RNase-Free centrifuge, onjezerani 60μL ya RDD buffer, ndikusakaniza pang'onopang'ono). Voliyumu yovomerezeka ya elution ndi 60μL. Ngati chitsanzocho sichinagayidwe mokwanira, chibwezereni ku gawo lachisanu kuti chigayidwenso. Kenako yesani motsatira malangizo ogwiritsira ntchito.