Mitundu 15 ya mphamvu yapamwamba kwambiri ya papillomavirus e6 / e7 free mrna
Dzina lazogulitsa
Mitundu ya HWTS-CC005A-15 ya mphamvu yapamwamba kwambiri ya papillomavirus E6 / E7 Gene Mrna Woona (Fluorescence PCR)
Epidemiology
Khansa yazemba ndi imodzi mwamitundu yodziwika bwino ya khansa yachikazi padziko lonse lapansi, ndipo kupezeka kwake kumakhala kogwirizana kwambiri ndi papillomavisis (HPV), koma gawo laling'ono la matenda a HPV amatha kukhala khansa. HPV yoopsa imadzetsa maselo a epithelical epithelical ndikupanga oncoproteins awiri, e6 ndi E7. Mapuloteni uyu amatha kusokoneza mapulongemi am'manja osiyanasiyana (monga proteins yopatuka prb ndi p53), amathandizira syna synthesis ndi bala lokhazikika, ndikusokoneza mayankho a antivirural.
Ngalande
| Ngalande | Chipangizo | Genotype kuyesedwa |
| Banja | Hpv | HPV16,31,35,51,52,58 |
| Vic / Hex | Gene β-Actin Gene | |
| Banja | Hpv zomwe zimapangitsa 2 | HPV 18,39,45,56,59,66,68 |
| Vic / Hex | Mtundu wa mtundu wa anthu |
Magawo aluso
| Kusunga | Madzi: ≤-18 ℃ |
| Alumali-Moyo | Miyezi 9 |
| Mtundu wofanana | Cervical swab |
| Ct | ≤38 |
| CV | <5.0% |
| Lodu | 500 makope / ml |
| Zida Zogwirizira | Ntchito za bioshasystems 7500 pcrImagwiritsa ntchito bioshasystems 7500 mwachangu pcr pcr systems Ma pcr-5 a PCR Slan-96P yeniyeni PCR Systems Speccycler®480 PCR System Stonegene 9600 kuphatikiza njira zenizeni za PCR Ma-6000 enieni ochulukirapo Biorad Cfx96 PCR PCR System Biorad cfx opus 96 yeniyeni PCR |
Kuyenda
Kulimbikitsidwa komwe kumalimbikitsa: Macro & Micro-Chef Crafl DNA / RNTS-3020-50-HPV15) ndi Meangsu . Makulidwe a Evel Evest ndi 50μl. Ngati chitsanzo sichikukulitsidwa kwathunthu, mubwezeretse mpaka Gawo 4 pokonzanso. Kenako yesani malinga ndi malangizo ogwiritsira ntchito.
Kulimbikitsidwa Kutulutsa: RASPREP yamphamvu ya nyama yokwanira RNA. Kutulutsa kuyenera kuchitidwa molingana ndi malangizo ogwiritsira ntchito (mu gawo 5, kawiri kawiri kuchuluka kwa dnase-free centrifuge chubu chatsopano, Onjezani 60. Mayunitsi olimbikitsidwa ndi 60μL. Ngati chitsanzo sichikukulitsidwa kwathunthu, mubwezeretse mpaka Gawo 5 kuti musinthe. Kenako yesani malinga ndi malangizo ogwiritsira ntchito.
