Mitundu 12 ya Matenda Oyambitsa Kupuma
Dzina la chinthu
HWTS-RT071A Mitundu 12 ya Kiti Yodziwira Matenda a Nucleic Acid (Fluorescence PCR)
Njira
| Njira | hu12Chida Chothandizira A | hu12Chida Chothandizira B | hu12Chida Chothandizira C | hu12Chida Chothandizira D |
| FAM | SARS-CoV-2 | HADV | HPIV Ⅰ | HRV |
| VIC/HEX | Kulamulira Kwamkati | Kulamulira Kwamkati | HPIV Ⅱ | Kulamulira Kwamkati |
| CY5 | IFV A | MP | HPIV Ⅲ | / |
| ROX | IFV B | RSV | HPIV Ⅳ | HMPV |
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Chidutswa cha m'kamwa |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | SARS-CoV-2: Makope 300/mLKachilombo ka chimfine B: Makopi 500/mLKachilombo ka fuluwenza A: Makope 500/mL Adenovirus: Makopi 500/mL mycoplasma pneumoniae: Makopi 500/mL Kachilombo ka respiratory syncytial virus: Makopi 500/mL, Kachilombo ka parainfluenza (Ⅰ, Ⅱ, Ⅲ, Ⅳ): Makopi 500/mL Kachilombo ka Rhinovirus: Makope 500/mL Matenda a pneumovirus a anthu: Makopi 500/mL |
| Kufotokozera Mwapadera | Kafukufuku wokhudza momwe zinthu zilili pa intaneti akuwonetsa kuti palibe njira yolumikizirana pakati pa zida izi ndi enterovirus A, B, C, D, kachilombo ka epstein-barr, kachilombo ka chikuku, kachilombo ka human cytomegalovirus, rotavirus, norovirus, kachilombo ka mumps, kachilombo ka varicella-herpes zoster, bordetella pertussis, streptococcus pyogenes, mycobacterium tuberculosis, aspergillus fumigatus, candida albicans, candida glabrata, pneumocystis jirovecii, cryptococcus neoformans ndi human genomic nucleic acid. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCRMachitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi YeniyeniQuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Makina a PCR a SLAN-96P a Nthawi Yeniyeni (Hongshi Medical Technology Co., Ltd.) Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time, LineGene 9600 Plus Real-Time PCR Detection System (FQD-96A, ukadaulo wa Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Njira 1.
Reagent yotulutsa yomwe ikulangizidwa: Macro & Micro-Test Sample Release Release Reagent (HWTS-3005-8) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Kuchotsa kuyenera kuchitika motsatira malangizo.
Njira yachiwiri.
Reagent yochotsera yomwe ikulangizidwa: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B) yochokera ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., kuchotsa kuyenera kuchitika motsatira malangizo mosamala. Voliyumu yovomerezeka ya elution ndi 80μL.
Njira 3.
Chotsukira chomwe chikulangizidwa: Nucleic Acid Extraction kapena Purification Kit (YDP315) chopangidwa ndi Tiangen Biotech(Beijing) Co., Ltd., kutsukira kuyenera kuchitika motsatira malangizo mosamala. Kuchuluka kwa kutsukira komwe kukulangizidwa ndi 100μL.
