Mitundu 11 ya Matenda Oyambitsa Kupuma
Dzina la chinthu
HWTS-RT162A Mitundu 11 ya Zida Zodziwira Matenda a Nucleic Acid Opumira(PCR ya Kuwala)
Njira
| 靶标 | Njira | |||
| 核酸反应液A | 核酸反应液B | 核酸反应液C | 核酸反应液D | |
| HI | Smet | MP | Mwendo | FAM |
| SP | PA | Bp | / | CY5 |
| KPN | ABA | Bpp | Cpn | ROX |
| 内参 | 内参 | 内参 | 内参 | VIC/HEX |
Epidemiology
Matenda opatsirana m'mapapo ndi gulu lofunika kwambiri la matenda omwe ali pachiwopsezo chachikulu pa thanzi la anthu, ndipo kafukufuku wasonyeza kuti matenda ambiri opatsirana m'mapapo amayamba chifukwa cha mabakiteriya ndi mavairasi omwe amafalikira pamodzi ndi munthu amene ali m'mapapo, zomwe zimapangitsa kuti matendawa achuluke komanso imfa, kotero kufotokoza bwino za matendawa kumathandiza kuti wodwalayo alandire chithandizo choyenera komanso kukulitsa moyo wake.[1,2].
Magawo aukadaulo
| Malo Osungirako | ≤-18℃ |
| Nthawi yokhalitsa | Miyezi 12 |
| Mtundu wa Chitsanzo | Kutupa kwa mbolo |
| Ct | HI、SP、KPN、PA、ABA、Smet：Ct≤33BP、Bpp、MP、Cpn、Mwendo：Ct≤38 |
| CV | <5.0% |
| LoD | Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Pseudomonasaeruginosa, Acinetobacter baumannii, Stenotrophomonas maltophilia ndi Legionellapneumophila: 1000 CFU/mL； Bordetella pertussis ndi Bacillus parapertussis: 500 CFU/mL; Mycoplasma pneumoniae ndi Chlamydia pneumoniae: makope 200/mL. |
| Kufotokozera Mwapadera | Zotsatira za mayeso obwerezabwereza zinasonyeza kuti panalibe kusinthana pakati pa zida izi ndi Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii ndi ma genomic nucleic acid a anthu. |
| Zida Zogwiritsidwa Ntchito | Machitidwe a Biosystems 7500 a Real-Time PCR Machitidwe a PCR Ogwiritsidwa Ntchito 7500 Ofulumira Nthawi Yeniyeni QuantStudio®Machitidwe 5 a PCR a Nthawi Yeniyeni Machitidwe a PCR a SLAN-96P a Nthawi Yeniyeni Woyendetsa Sitima Yopepuka®Dongosolo la PCR la 480 Real-Time Makina Ozindikira a PCR a LineGene 9600 Plus Real-Time MA-6000 Real-Time Quantitative Thermal Cycler Dongosolo la PCR la BioRad CFX96 Real-Time BioRad CFX Opus 96 Real-Time PCR System |
Kuyenda kwa Ntchito
Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (yomwe ingagwiritsidwe ntchito ndi Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) yopangidwa ndi Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Onjezani 200µL yathupiThirani mchere ku precipitate yokonzedwa, ndipo njira zotsatirazi ziyenera kuchitika motsatira malangizo. Kuchuluka kwa elution komwe kumalimbikitsidwa ndi 100 µL.
